Fig 6.

Estimation of parasite burden in the spleen and liver of the experimental group of animals: (A) Liver and (B) Spleen. Total parasite load in liver and spleen was estimated by the microscopic evaluation of Giemsa stained tissue from the corresponding organs and is expressed in LDU (Leishman-Donovan) units; where LDU = number of amastigote per nucleated cell x organ weight in milligram. The results are the mean±SD of 5 animals per group and unpaired two-tailed Student’s t-test was performed to determine the levels of significance indicated by P values. Treatment with (+) and without (-) suramin is indicated in the box below. At a dose of 20mg/kg/day (twice a week for 15 days) there are significant reductions in the hepatic (A) and splenic (B) parasitic loads respectively.