Fig 8. Assessment of TH1 and TH2 cytokine productions in all the experimental group of animals (Normal, ONLY TRE, INF,INF+TRE).
TH1 and TH2 cytokine generation assayed by ELISA performed on the supernatants collected from mice splenocytes pulsed with SLA, from the different groups (given in the boxes below the bar diagrams) for (A) IFN-gamma (B) TNF-alpha, (C) IL-12, (D) IL-10, (E) TGF-beta cytokine levels. Unpaired two-tailed Student’s t-test was performed and levels of significance indicated by P values with NS denoting statistical insignificance. The results show a significant increase of pro-inflamatory TH1 cytokines in INF+TRE relative to INF, with a decrease in TH2 cytokines indicative of recovery from infection.