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. 2020 Sep 15;15(9):e0238616. doi: 10.1371/journal.pone.0238616

Fig 1. Each domain of Pikp-1 and Pikp-2 is required for receptor activation.

Fig 1

A) Representative N. benthamiana leaves showing that expression of the individual domains of Pikp-1 (left) or Pikp-2 (right) were unable to elicit a cell death response in presence of the corresponding paired NLR (for Pikp-1) or paired NLR and effector (for Pikp-2). Pikp-1+Pikp-2+AVR-PikD is shown as a positive control. B) Representative agroinfiltration spots show that the truncated variants of Pikp-1 (left) or Pikp-2 (right) were unable to elicit a cell death response, either when overexpressed alone, or in the presence of corresponding full-length NLR and/or effector. Combinations of constructs without HMA domain were not tested (N/T) in presence of the effector.