Table 5. Comparison of the amino acid residues of E gene among 145 samples.

Amino Acid Residue	Domain	SA 14-14-2	P3	Beijing-1	3 GIII strainsa	139 GI samplesb
E76	II	T	M	T	T	T
E107*	II	F	L	L	L	L
E129	II	T	T	T	T	M
E132	II	P	P	S	P	P
E138*	I	K	E	E	E	E
E176*	I	V	I	I	I	I
E177*	I	A	T	T	T	T
E222	II	A	A	A	A	S
E227	II	S	P	P	S	S
E244	II	G	E	E	E	E
E264*	II	H	Q	Q	Q	Q
E279*	II	M	K	K	K	K
E306	I	E	G	E	E	E
E315*	III	V	A	A	A	A
E327	III	S	S	S	S	T
E366	III	A	A	A	A	S
E397	III	H	H	Y	H	H
E408	III	S	L	S	S	S
E439*	Non	R	K	K	K	K
E447	Non	D	G	G	G	G
E473	Non	V	V	I	V	V

Note

*indicates the eight key amino acid residues related to neuroinvasiveness and neurovirulence attenuation in E gene.

^a indicates the 3 isolated GIII JEV strains in Zhejiang Province in 1982–1983.

^b indicates the 139 JEV-positive sequencing samples (GI) in Zhejiang Province in 2015–2018.