Fig 3. Manganese Enhanced MR Imaging (MEMRI) of the intraosseous human breast cancer xenotransplant model and CaSR/TRPV6 receptors levels.

A. Tumor diameter 10 mm. On T2-weighted MR image a low signal intensity solid tumor is detected at the left leg of the mouse (red arrow). BC. On T1-weighted gradient echo MR images (T1 WI) recorded before and 90 minutes after Mn²⁺ administration, no changes in tumor enhancement was appreciated (red arrows). D. X-Ray imaging of the left leg of the mouse showed multiple lytic areas within the tibia in keeping with the tumor transplantation (red circle). E. Hematoxylin and eosin staining displaying the intraosseous tumor. FG. Immunohistochemistry (IHC) of CaSR and TRPV6: both receptors were not detected in tumor cells (score 0) showing a negative staining. Scale bar = 100μ.