Fig 4. Manganese Enhanced MR Imaging (MEMRI) of the pseudometastatic prostate cancer animal model and CaSR/TRPV6 receptors levels.

A. Multiple tumours with diameter from 3 to 10 mm. T2-weighted MR images showed multiple high intensity nodules with intra-abdominal ascites. B-Q. T1-weighted gradient echo MR images (T1 WI) recorded before and 90 minutes after Mn²⁺ administration: B-G. No manganese uptake by the liver metastases was detected (red arrows); L-Q. An increase in signal enhancement after manganese administration, respectively, within a diaphragmatic (LM) and mediastinal nodules (PQ) is appreciated (red arrows). D-S. Immunohistochemistry (IHC) of CaSR and TRPV6: D-I. A rare positive staining of CaSR was detected in tumour cells (score 1), while TRPV6 receptors displayed intense staining (score 4); N-S. Non-uniform weak/intense expression of CaSR was detected in tumour cells (score 3), while TRPV6 receptors ranged from strong uniform (score 4) to rare positive (score 2). Scale bar = 100μ.