Fig. 2.

The 96-well microplate lay-out for quantification of hydrogen peroxide in honey samples. Each honey sample requires 12 wells divided into quadrants with three replicates in each quadrant. Quadrants consist of honey alone and three honey-containing controls. This scheme allows for up to five honey samples and catalase controls to be assayed (in triplicate) on a single plate. The diagonal line in column 12 indicates empty assay plate wells.