Figure S1.

Structural Determination of Spike D614G, Related to Figure 6

(A) Size exclusion column elution profiles of D614G (blue line) and D614 (red line).

(B) Coomassie-stained SDS-PAGE of the peaks collected in (A) shows equivalent, full-length monomeric S proteins at around 180 kD.

(C) A still frame from a raw micrograph movie. Individual particles can be clearly visualized.

(D) 2D-clustering of trimeric D614G particles. Dagger denotes a top-view. Asterisk denotes a side-view.

(E) Fourier shell correlation diagram for D614G (unmasked).

(F) Half map Fourier shell correlation diagram (unmasked).

(G) Model to map Fourier shell correlation diagram.

(H) Local resolution of the ensemble map for Spike D614G.