Table 1.
Summary of Cryo-EM Data Collection, Three-Dimensional Reconstruction, and Model Refinement
| SARS-Covid19 Spike (D614G) | |
|---|---|
| Imaging Parameters and Three-Dimensional Reconstruction | |
| Cryo-EM grids | UltrAuFoil R1.2/1.3 |
| Calibrated magnification | 155,000 |
| Acceleration voltage [kV] | 300 |
| Pixel size [Å] | 0.52 |
| Total dose [e−/Å2] | 37 |
| Exposure time [s] | 1.81 |
| Defocus range [μm] | −0.6 ∼−1.8 |
| Particles in final 3D reconstruction | 266,356 |
| Resolution (“Gold-standard” at FSC 0.143) [Å] | 3.7 |
| Model Refinement | |
| Resolution in phenix.real_space_refine [Å] | 4.0 |
| No. of atoms: protein | 18,294 |
| Ligands/ions | N/A |
| rmsds: bond lengths [Å] | 0.004 |
| rmsds: bond angles [°] | 0.808 |
| MolProbity score | 4.49 |
| Rotamer outliers [%] | 0.00 |
| Ramachandran angles: favored [%] | 93.88 |
| Ramachandran angles: allowed [%] | 6.12 |
| Ramachandran angles: outliers [%] | 0.00 |
| PDB | 6XS6 |
| EMDB | EMD-22301 |