REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal anti-SARS-CoV-2 M protein	ThermoFisher	PA1-41160; RRID: AB_1087198
Rabbit polyclonal anti-SARS-CoV-2 E protein	Antibodies-online.com	ABIN1031551
Rabbit polyclonal anti-SARS-CoV-2 N protein	Rockland Immunochemicals	200-401-A50; RRID: AB_828403
Rabbit monoclonal anti-SARS-CoV-2 S protein (S1-specific)	GeneTex	GTX635656
Rabbit monoclonal anti-SARS-CoV-2 S protein (S1-specific)	GeneTex	GTX635672
Mouse/human monoclonal anti-SARS-CoV-2 S protein (S2-specific)	Sino Biological	40590-D001; RRID: AB_2857932
Rabbit polyclonal anti-Raptor	EMD Millipore	09-217; RRID: AB_612103
Goat anti-rabbit antibody 800CW	Li-Cor	925-32211; RRID: AB_2651127
Goat anti-mouse 680RD	Li-Cor	925-68070; RRID: AB_2651128
Goat anti-rabbit, HRP-linked antibody	Cell Signaling Technology	7074; RRID: AB_2099233
Goat anti-mouse, HRP-linked antibody	Cell Signaling Technology	7076; RRID: AB_330924
Human monoclonal antibody targeting the receptor binding domain of the SARS-CoV-2 S protein	A. Baum et al., Science10.1126/science.abd0831 (2020)J. Hansen et al., Science10.1126/science.abd0827 (2020)	REGN10987
Human monoclonal antibody targeting the receptor binding domain of the SARS-CoV-2 S protein	A. Baum et al., Science10.1126/science.abd0831 (2020)J. Hansen et al., Science10.1126/science.abd0827 (2020)	REGN10989
Human monoclonal antibody targeting the receptor binding domain of the SARS-CoV-2 S protein	A. Baum et al., Science10.1126/science.abd0831 (2020)J. Hansen et al., Science10.1126/science.abd0827 (2020)	REGN10933
Human monoclonal antibody targeting the receptor binding domain of the SARS-CoV-2 S protein	A. Baum et al., Science10.1126/science.abd0831 (2020)J. Hansen et al., Science10.1126/science.abd0827 (2020)	REGN10934
Mouse monoclonal, anti-human Fc antibody	A. Baum et al., Science10.1126/science.abd0831 (2020)J. Hansen et al., Science10.1126/science.abd0827 (2020)	REGN2567
Bacterial and Virus Strains
VSVΔG:mNeon/VSV-G virus	A. Baum et al., Science10.1126/science.abd0831 (2020)J. Hansen et al., Science10.1126/science.abd0827 (2020)	N/A
Chemicals, Peptides, and Recombinant Proteins
hsACE2.hFc	A. Baum et al., Science10.1126/science.abd0831 (2020)J. Hansen et al., Science10.1126/science.abd0827 (2020)	N/A
blasticidin	InvivoGen	ant-bl-1
puromycin	InvivoGen	ant-pr-1
gentamicin	Life Technologies	15750078
Ni-NTA resin	EMD Millipore	30230
Superose 6 gel-filtration column	GE Healthcare	29091596
PEI	Polysciences, Inc.	23966-1
TransIT LT1 transfection reagent	Mirus Bio	MIR 2304
Lipofectamine LTX	Life Technologies	15338100
Cytofix	BD Biosciences	554655
SMM-293 TII serum-free media	SinoBiological	M293TI
Critical Commercial Assays
Mycoplasma detection kit	Lonza	LT07-318
Steady-Glo Luciferase Assay System	Promega	E2550
Bradford Protein Assay	Bio-Rad	5000001
Deposited Data
PDB	6xs6	N/A
EMDB	EMD-22301	N/A
Experimental Models: Cell Lines
Human: HEK293 cells	ATCC	CRL-1573; RRID: CVCL_0045
Human: HEK293T cells	ATCC	CRL-3216; RRID: CVCL_0063
Human: HEK293T/17 cells	ATCC	CRL-11268; RRID: CVCL_1926
Human: FreeStyle 293-F cells	ThermoFisher	R79007
Human: Calu-3 cells	ATCC	HTB-55; RRID: CVCL_0609
Human: Caco-2 cells	ATCC	HTB-37; RRID: CVCL_0025
Human: SUPT1 [VB] cells	ATCC	CRL-1942; RRID: CVCL_1714
Cercophithecus aethiops: Vero cells	ATCC	CCL-81; RRID: CVCL_0059
Recombinant DNA
pcDNA3.1 SARS-CoV-2 S D614	This publication	Addgene #158074
pcDNA3.1 SARS-CoV-2 S D614G	This publication	Addgene #158075
pcDNA3.1 SARS-CoV-2 S D614-trunc	This publication	Addgene #158076
pcDNA3.1 SARS-CoV-2 S D614G-trunc	This publication	Addgene #158077
pcDNA3.1 SARS-CoV-2 M	This publication	Addgene #158078
pcDNA3.1 SARS-CoV-2 N	This publication	Addgene #158079
pcDNA3.1 SARS-CoV-2 E	This publication	Addgene #158080
pscALPSpuro	Addgene	Cat# 128504
pscALPSpuro-HsACE2 (human)	This publication NP_001358344.1	Addgene #158081
pscALPSpuro-RsACE2 (Chinese rufous horseshoe bat)	This publication ACT66275	Addgene #159665
pscALPSpuro-FcACE2 (Cat)	This publication XP_023104564.1	Addgene #158082
pscALPSpuro-ClACE2 (Dog)	This publication NP_001158732.1	Addgene #158083
pscALPSpuro-MjACE2 (Pangolin)	This publication XP_017505752	Addgene #158084
pscALPSblasti-GFP	Addgene	Cat# 115804
pCSDest TMPRSS2	Addgene	Cat# 53887
pscALPSblasti-TMPRSS2 Blasti	This publication	Addgene #158088
HIV-1 pNL4-3 Δenv Δvpr luciferase reporter vector (pNL4-3.Luc.R-E-)	NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH: from Dr. Nathaniel Landau	ARP Cat #3418
pUC57mini NL4-3 Δenv eGFP	NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH: this publication	ARP Cat #13906
psPAX2	Addgene	Cat #12260
pMD2.G	Addgene	Cat# 12259
pCAGGS-ACE2-his	This publication	Addgene #158089
Software and Algorithms
FlowJo	FlowJo LLC; BD	N/A
Prism 8.4.3	GraphPad	N/A
T200 Evaluation software v 3.1	Biacore	N/A
Scrubber 2.0	BioLogic Software	N/A
Coot	https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/	Version 0.9
PHENIX	https://www.phenix-online.org/	Version 1.18.2
MolProbity	http://molprobity.biochem.duke.edu/	Version 4.5.1
MAFFT v7.464	Katoh and Standley, 2013	https://mafft.cbrc.jp/alignment/software/
Biopython v1.76	Cock et al., 2009	https://biopython.org/
Matplotlib v3.2.2	Hunter, 2007	https://matplotlib.org/
SnpEff v4.5covid19	Cingolani et al., 2012	http://snpeff.sourceforge.net/
bcftools v1.9	Li et al., 2009	http://samtools.github.io/bcftools/bcftools.html
snp-sites v2.5.1	Page et al., 2016	https://sanger-pathogens.github.io/snp-sites/
EPU 2 software for single particle analysis	Thermo Fisher Scientific	N/A
Other
Quantifoil Au grid	Quantifoil, Großlöbichau Germany	N/A
GloQube Plus Glow Discharge System	Quorumtech	N/A
Vitrobot Mark IV	Thermo Fisher Scientific	N/A
Krios Rx Cryo-transmission electron microscope with 300 kV fixed voltage	Thermo Fisher Scientific	N/A