Table 1. Binding affinities of PHF1/MTF2 mutants with the H3tK27me3 peptide.
PHF1 | ITC (µM) |
---|---|
W41A | NB |
Y47A | NB |
F65A | NB |
F71A | 192 ± 18 |
L38A | 125 ± 9 |
L45A | 178 ± 9 |
L46A | 127 ± 8 |
L46Y | 50 ± 5 |
L48A | 81 ± 2 |
L38A, L45A, L46A, L48A | >500 |
E66A | >200 |
MTF2 | ITC (µM) |
S86F | 91 ± 21 |
S86Y | 110 ± 11 |
NB indicates no detectable binding.