Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (S. cerevisiae) | MATa ura::ADH1 promoter-OsTIR1-9myc::URA3 Scc3-PK3-aid::KanMX4 SCC1-HA3::HIS3 | This study | KN20783 | |
| Strain, strain background (S. cerevisiae) | MATa Scc3-PK3-aid::KanMX4 SCC1-HA3::HIS3 | This study | KN20785 | |
| Strain, strain background (S. cerevisiae) | MATa/alpha scc3::NatMX4/WT | This study | KN21079 | |
| Strain, strain background (S. cerevisiae) | MATa/alpha scc3::NatMX4/WT, leu::Scc3-HA3::LEU | This study | KN21273 | |
| Strain, strain background (S. cerevisiae) | MATa Scc1-PK9::KanMX scc2-45::natMX (L545P D575G) | This study | KN22390 | |
| Strain, strain background (C. glabrata) | MATa, SCC1-PK9::NATMX4 | Petela et al., 2018 | KN23308 | |
| Strain, strain background (S. cerevisiae) | MATa Scc1-PK9::KanMX scc2-45::natMX (L545P D575G) lys2::Scc2-HyGMX | This study | KN24185 | |
| Strain, strain background (C. glabrata) | MATa, SCC1-HA3::NATMX4 | Petela et al., 2018 | KN25532 | |
| Strain, strain background (S. cerevisiae) | MATa Scc1-PK9::KanMX scc2-45::natMX (L545P D575G) LYS2::Scc2(S717L,K721E)-HygMX | This study | KN27010 | |
| Strain, strain background (S. cerevisiae) | MATa/alpha scc3::NatMX4/WT, leu::Scc3 (K224E, K225E, R226E)-HA3::LEU | This study | KN27539 | |
| Strain, strain background (S. cerevisiae) | MATa scc3::NatMX4, Scc1-PK6::TRP1, leu::Scc3-HA3::LEU | This study | KN27542 | |
| Strain, strain background (S. cerevisiae) | MAT alpha scc3::NatMX4, Scc1-PK6::TRP1, leu::Scc3 (K224E, K225E, R226E)-HA3::LEU | This study | KN27547 | |
| Strain, strain background (S. cerevisiae) | MATa/alpha scc3::NatMX4/WT, leu::Scc3 (K423E, K513E, K520E)-HA3::LEU | This study | KN27696 | |
| Strain, strain background (S. cerevisiae) | MATa scc3::NatMX4, Scc1-PK6::TRP1, leu::Scc3 (K423E, K513E, K520E)-HA3::LEU | This study | KN27697 | |
| Strain, strain background (S. cerevisiae) | MATa/alpha scc3::NatMX4/WT, leu::Scc3 (K224E, K225E, R226E, K423E, K513E, K520E)-HA3::LEU | This study | KN27763 | |
| Strain, strain background (S. cerevisiae) | MATa ura::ADH1promoter-OsTIR1-9myc::URA3, Scc3-PK3-aid::KanMX4, leu::Scc3-HA3::LEU | This study | KN27796 | |
| Strain, strain background (S. cerevisiae) | MATa ura::ADH1 promoter-OsTIR1-9myc::URA3, Scc3-PK3-aid::KanMX4 leu::Scc3 (K224E, K225E, R226E, K423E, K513E, K520E)-HA3::LEU | This study | KN27802 | |
| Strain, strain background (S. cerevisiae) | MATa ura::ADH1promoter-OsTIR1-9myc::URA3, Scc3-HA3-aid::KanMX4, Scc1-PK6::TRP1, leu::Scc3 (K224E, K225E, R226E, K423E, K513E, K520E)-HA3::LEU | This study | KN27804 | |
| Strain, strain background (S. cerevisiae) | MATa ura::ADH1promoter-OsTIR1-9myc::URA3, Scc3-HA3-aid::KanMX4, Scc1-PK6::TRP1, leu::Scc3-HA3::LEU | This study | KN27821 | |
| Strain, strain background (S. cerevisiae) | MATa ura::ADH1promoter-OsTIR1-9myc::URA3, Scc3-HA3-aid::KanMX4, leu::Scc3-HA3::LEU, Scc2-PK9::NatMX | This study | KN28075 | |
| Strain, strain background (S. cerevisiae) | MATa ura::ADH1 promoter-OsTIR1-9myc::URA3, Scc3-HA3-aid::KanMX4, Scc2-PK9::NatMX, leu::Scc3 (K224E, K225E, R226E, K423E, K513E, K520E)-HA3::LEU | This study | KN28287 | |
| Strain, strain background (S. frugiperda) | Sf9 insect cells | ThermoFisher | Cat# 11496015 | |
| Antibody | Anti-His (mouse) | Sigma | Cat# SAB1305538-400UL | 1:2000 |
| Antibody | Anti-mouse HRP | ThermoFisher | Cat# 62–6520 | 1:5000 |
| Antibody | Anti-Smc3 (mouse) | Bethyl Laboratories | Cat# A300-060A | 1:500 |
| Antibody | Anti-Strep HRP | iba | Cat# 2-1502-001 | 1:4000 |
| Recombinant DNA reagent | pACEbac1 SMC1-His | This Study | ||
| Recombinant DNA reagent | pACEbac1 SMC3 | This Study | ||
| Recombinant DNA reagent | pACEbac1 SMC1-His SMC3 | This Study | ||
| Recombinant DNA reagent | pACEbac1 Scc2133-1493-2xStrepII | This Study | ||
| Recombinant DNA reagent | pACEbac1 SCC2-2xStrepII | This Study | ||
| Recombinant DNA reagent | pACEbac1 2xStrepII-Scc2151-1493 | This Study | ||
| Recombinant DNA reagent | pACEbac1 2xStrepII-SCC3 | This Study | ||
| Recombinant DNA reagent | pIDC SCC1-2xStrepll | This Study | ||
| Recombinant DNA reagent | pIDC Scc1269-451-2xStrepII | This Study | ||
| Recombinant DNA reagent | pIDC Scc1150-298-2xStrepII | This Study | ||
| Recombinant DNA reagent | pIDC His-SCC4 | This Study | ||
| Chemical compound, drug | ATP Lithium Salt | Sigma | Cat# 11140965001 | |
| Chemical compound, drug | Bismaleimidoethane (BMOE) | ThermoFisher | Cat# 22323 | |
| Chemical compound, drug | Complete EDTA free protease inhibitor cocktail | Roche | Cat# 4693132001 | |
| Chemical compound, drug | Cre Recombinase | New England Biolabs | Cat# M0298S | |
| Chemical compound, drug | Desthiobiotin | Fisher Scientific | Cat# 12753064 | |
| Chemical compound, drug | EtBr | ThermoFisher | Cat# 15585011 |
|
| Chemical compound, drug | Fetal Bovine Serum | Sigma | Cat# 12303C | |
| Chemical compound, drug | FuGENE HD Transfection reagent | Promega | Cat# E2311 | |
| Chemical compound, drug | Gibson Assembly Mix | New England Biolabs | Cat# E2611L | |
| Chemical compound, drug | Immobilon Western ECL | Millipore | Cat# WBLKS0500 | |
| Chemical compound, drug | NuPAGE 3–8% Tris-Acetate Protein Gels | ThermoFisher | Cat# EA0378BOX | |
| Chemical compound, drug | PMSF | Sigma | Cat# 329-98-6 | |
| Chemical compound, drug | Quick Coomassie Stain | Generon | Cat# GEN-QC-STAIN | |
| Chemical compound, drug | RNase A | Roche | Cat# 10109169001 | |
| Chemical compound, drug | Sf900 II SFM | ThermoFisher | Cat# 10902104 | |
| Chemical compound, drug | Supernuclease | SinoBiological | Cat# SSNP01 | |
| Chemical compound, drug | TCEP | ThermoFisher | Cat# 20490 | |
| Chemical compound, drug | 4xLDS | ThermoFisher | Cat# NP0007 | |
| Commercial assay or kit | HiLoad 16/60 Superdex 200 | GE Healthcare | Cat# GE28-9893-35 | |
| Commercial assay or kit | HiSpeed Plasmid Maxi Kit | Qiagen | Cat# 12663 | |
| Commercial assay or kit | HiTrap Q HP | GE Healthcare | Cat# GE29-0513-25 | |
| Commercial assay or kit | StrepTrap HP | Fisher Scientific | Cat# 11540654 | |
| Commercial assay or kit | Superose 6 Increase 10/300 GL | VWR | Cat# 29-0915-96 | |
| Commercial assay or kit | EnzChek phosphate assay kit | Invitrogen | Cat# E6646 | |
| Software, algorithm | RELION 3.1 | doi:10.1016/j.jsb.2012.09.006 | ||
| Software, algorithm | CtfFind4 | doi:10.1016/j.jsb.2015.08.008 | ||
| Software, algorithm | Warp | doi:10.1038/s41592-019-0580-y | ||
| Software, algorithm | CrYOLO 1.5 | doi:10.1038/s42003-019-0437-z | ||
| Software, algorithm | Chimera | https://www.cgl.ucsf.edu/chimera/ | ||
| Software, algorithm | ChimeraX 1.0 | https://www.cgl.ucsf.edu/chimerax/ | ||
| Software, algorithm | COOT | doi:10.1107/S0907444910007493 | ||
| Software, algorithm | MAIN | doi:10.1107/S0907444913008408 | ||
| Software, algorithm | Phenix.real_space_refinement | doi:10.1107/S2059798318006551 | ||
| Software, algorithm | PYMOL 2 | https://pymol.org/2/ | ||
| Software, algorithm | SWISS-MODEL | https://swissmodel.expasy.org | ||
| Other | Quantifoil Au 2/2 holely carbon 200 mesh cryoEM grids | Quantifoil GmbH | ||
| Other | Ultrafoil 2/2 holely gold 200 mesh cryoEM grids | Quantifoil GmbH |