Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Lobophyllia hemprichii) | mEos3.1 | FPbase | FPbase:73GT1 | |
| Recombinant DNA reagent | pRSET_His-rsCaMPARI-mRuby3 (plasmid) | This paper | RRID:Addgene_120804 | Plasmid available at Addgene |
| Recombinant DNA reagent | pAAV-hsyn_NES-His-rsCaMPARI-mRuby3 (plasmid) |
This paper | RRID:Addgene_120805 | Plasmid available at Addgene |
| Recombinant DNA reagent | pAAV-hsyn_NLS-His-rsCaMPARI-mRuby3 (plasmid) | This paper | RRID:Addgene_122092 | Plasmid available at Addgene |
| Recombinant DNA reagent | pTol2-elavl3_NES-rsCaMPARI-mRuby3 (plasmid) | This paper | RRID:Addgene_122129 | Plasmid available at Addgene |
| Recombinant DNA reagent | pAAV-hsyn_ChrimsonR-HaloTag (plasmid) | PMID:24509633 and PMID:18533659 | ||
| Strain, strain background (Escherichia coli) | T7 express | New England Biolabs | C2566 | Competent cells |
| Biological sample (Rattus norvegicus) | Primary rat hippocampal neurons | Janelia Research Campus | Freshly isolated from Rattus norvegicus | |
| Genetic reagent (Danio rerio) | Tg[elavl3:rsCaMPARI-mRuby3]jf93 | This paper | jf93Tg/+ | ZFIN ID: ZDB-FISH-191008–1 |
| Chemical compound, drug | Synaptic blockers | Tocris; PMID:24155972 | Cat# 0190, 0247, 1262, 0337 | |
| Chemical compound, drug | Janelia Fluor 635 dye, HaloTag ligand | PMID:28924668 | Luke Lavis’ lab | |
| Chemical compound, drug | Tricaine methanesulfonate (MS-222) | Sigma | E10521 | |
| Chemical compound, drug | 4-Aminopyridine | Sigma | 275875 | |
| Software, algorithm | CMTK | PMID:12670015 | RRID:SCR_002234 |