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| Ca2+ | λabs (nm) | λex (nm) | ϵ (M−1cm−1) | ΦF | Brightness* | ↓ rate† (s−1) | ↑ rate‡ (s−1) | Kd (nM) | Hill coefficient | |
|---|---|---|---|---|---|---|---|---|---|---|
| rsCaMPARI (on) | - | 502 | 515 | 60304 | 0.54 | 32.6 | 0.028 ± 0.002 | - | 167 ± 6 | 3.96 |
| + | 500 | 515 | 38234 | 0.46 | 17.6 | 0.467 ± 0.066 | - | |||
| rsCaMPARI (off) | - | 392 | - | - | - | - | - | 4.37 ± 0.24 | - | - |
| + | 389 | - | - | - | - | - | 6.40 ± 0.28 |
*Brightness = ϵ x ΦF / 1000.
†Measured during irradiation with 200 mW/cm2 of 484 nm light.
‡Measured during irradiation with 200 mW/cm2 of 405 nm light.
