Fig. 2.

NFs decreased ccRCC cells proliferation and increased migration without EMT. a CcRCC cells were cultured for 72 h in the presence or absence of NFs (NF170829, NF170831, NF170923, NF171006, NF171130, respectively) conditioned media. CCK-8 test was performed to evaluate the proliferation ability of different ccRCC cells (786-O, 769-P, ACHN, Caki-1). *P < 0.05 vs. 1640. b Transwell assays were carried out to detect cells migration ability. Representative photographs are presented in ×200 magnification. *P < 0.05 vs. 1640. c CcRCC cells were cultured for 24 h in the presence or absence of five different NF-CMs, respectively. Several markers associated with the EMT process, such as α-SMA and vimentin were detected by western blotting analysis. d The mRNA expression of α-SMA and vimentin were detected by qRT-PCR. All results did not have any statistical significance. Note that the addition of the NF-CM weakened the proliferation and enhanced the migration of the ccRCC cells, but (the addition of the NF-CM) did not induce EMT