Fig. 3.

The cytokines secretion and STAT3 activation after the co-culture of NF-CM and ccRCC cells. CcRCC cells were cultured for 72 h in the presence or absence of different NF-CMs. a Expression levels of IL6, VEGF, IL1β, and CXCL12 in media were measured using ELISA, respectively. IL6 levels were remarkably increased in all cells, while VEGF was only increased in ACHN cells. CXCL12 secretion was increased in 786-O and 769-P cells, but IL1β secretion was decreased. *P < 0.05, **P < 0.01, ***P < 0.005 vs. 1640. b Protein expression of p-STAT3 and total STAT3 were detected by western blotting, and representative results from one of the three independent experiments are presented. *P < 0.05, **P < 0.01, ***P < 0.001 vs. 1640. Note that NF-CM induced expression of IL6, VEGF and CXCL12 increased, decreased that of IL1β and activated STAT3 in ccRCC cells