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. 2020 Sep 1;40(5):852–859. doi: 10.5851/kosfa.2020.e51

Fig. 1. Purification of pig muscle stem cells by magnetic-activated cell sorting (MACS) using a CD29 antibody.

Fig. 1.

Muscle stem cells isolated from the biceps femoris muscle of 14 d-old LYD pigs were sorted by MACS using a CD29 antibody. (A) The expression pattern of CD29 and CD56 in pig muscle stem cells as determined by immunostaining. Blue, green, and red fluorescence represent nuclei, CD29, and CD56, respectively. (B) The proportion of CD29- and CD56-positive cells in pig muscle stem cells as measured by immunostaining. (C) The proportion of CD29- and CD56-positive cells following MACS sorting was measured by immunostaining in cells from the various breeds (LYD, Berkshire, and Korean native pigs). Data are represented as mean±SEM. The significance of the differences was determined between the unsorted and sorted groups. * p<0.05, ** p<0.01, and *** p<0.001. Scale bar=200 μm. LYD, Landrace×Yorkshire×Duroc; CD, cluster of differentiation.