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. 2020 Jun 29;123(6):955–964. doi: 10.1038/s41416-020-0965-9

Fig. 4. Coexistence of EP2 and syntenin-1 is associated with L-OHP chemosensitivity and sphere formation of cancer.

Fig. 4

a PTGER2 expression levels in SW480, SW620 and Caco2 cells transfected with negative control siRNA or siRNAs against PTGER2. Reverse transcription-quantitative polymerase chain reaction was performed to detect mRNA expression levels, which were normalised to ACTB. Results represent the mean ± SD of three independent experiments. *p < 0.05. b Chemosensitivity assay of cells transfected with siRNA targeting PTGER2 expression. Chemosensitivity was indicated by viability after treatment with L-OHP for 72 h. Results represent the mean ± SD of three independent experiments. *p < 0.05. c Sphere formation by cells transfected with siRNA targeting PTGER2 expression. Lower panel, representative images of a sphere. Scale bar, 50 µm. *p < 0.05. d Chemosensitivity assay of cells transfected with shRNA targeting SDCBP expression with/without prostaglandin E2 or celecoxib. Chemosensitivity was indicated by viability after treatment with L-OHP for 72 hours. Results represent the mean ± SD of three independent experiments. *p < 0.05. e Sphere formation by cells transfected with shRNA targeting SDCBP expression with/without prostaglandin E2 or celecoxib. Lower panel, representative images of a sphere. Scale bar, 50 µm. *p < 0.05.