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. 2020 Sep 15;11(9):756. doi: 10.1038/s41419-020-02948-2

Fig. 4. DOX induces ferroptotic-like cell death in HL-1 cardiomyocytes and decreases Acot1 expression.

Fig. 4

a HL-1 Cell viability analysis of the protective effect of Fer-1 (10 μM) in different concentrations of RSL-3-induced cell death for 24 h. b HL-1 Cell viability analysis of the protective effect of Fer-1 (10 μM) in different concentrations of DOX-induced cell death for 24 h. c Relative intracellular GSH/GSSG levels in RSL-3 (5 μM) or DOX (2 μM) treated HL-1 cells with or without Fer-1 (10 μM) for 6 h. d Ptgs2 mRNA level in different concentrations of DOX-treated HL-1 cell with or without Fer-1 (10 μM) for 24 h. e Flow cytometer analysis of C11-BODIPY 581/591 staining showing lipid peroxidation level in RSL-3 (5 μM) or DOX (2 μM) treated HL-1 cells with or without Fer-1 (10 μM) for 6 h. f Acot1 mRNA levels in different concentrations of DOX-treated HL-1 cell with or without Fer-1 (10 μM) for 24 h. g Acot1 protein levels in different concentrations of DOX-treated HL-1 cell with or without Fer-1 (10 μM) for 24 h. Significance in a-d, f, g was calculated using the unpaired Student’s t test. P value < 0.05 was considered to be significant, and labeled as *P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.001; ns not significant.