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Nuclear exit of HDACs1/8/10 requires signaling by the AMPK. HT1080 cells were transfected with a scrambled siRNA (siSCR) or with an siRNA to knock down the expression of AMPKα. Twenty-four hour afterwards, cells treated with vehicle control or with [GZ17-6.02 (2 μM final curcumin)] 1 h. Cells were fixed in place and immunostaining performed to detect the expression and localization of HDACs1/8/10.
