Figure 2.
Antitumor effect of SAM and anti-PD-1 antibody in a syngeneic mouse B16-F1 melanoma model in vivo. 5 × 105 B16-F1 mouse melanoma cells were injected via the subcutaneous route into the right flank of C57BL/6 mouse (n = 8/group). From day 3 post tumor cells, inoculation mice were treated with control IgG alone (control), 80 mg/kg SAM, 10 mg/kg anti-PD-1 antibody, and SAM+anti-PD-1 antibody as described in Materials and Methods. (A) Tumor volume was measured at days 12, 14, and 16. All control and experimental mice were sacrificed on day 16. (B) Percentage (%) of tumor volume reduction in each group relative to control at day 16. (C) Tumor weight was measured after sacrifice on day 16. (D) Mean mouse body weights measured at different time intervals (days 1, 12, 14, and 16) for each group. (A–D) Results are representative of mean ± SEM of at least 8 mice per group. Statistical significance was obtained by ANOVA in GraphPad prism and are represented by asterisks (ns, not significant, *P < 0.05, **P < 0.01, and ****P < 0.0001).