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. 2020 Jun 30;123(6):1012–1023. doi: 10.1038/s41416-020-0929-0

Fig. 3. WDR1 knockdown suppresses tumour growth and metastasis of pancreatic cancer.

Fig. 3

a Western blotting analysis of WDR1 protein expression in PDAC cells. b The shRNA-mediated WDR1-knockdown efficiency in PANC1 and AsPC1 cells was analysed by Western blotting. c The effect of WDR1 knockdown on in vitro PDAC cell proliferation was determined by plate colony-formation assay. d The effect of WDR1 knockdown on in vivo PDAC tumour growth was studied by PANC1-derived subcutaneous xenograft model. e The tumour volume and weight of sh-Ctrl and sh-WDR1 PANC1-derived subcutaneous xenograft tumour tissues. f Representative immunohistochemical images of Ki67 staining from sh-Ctrl and sh-WDR1 PANC1-derived tumour tissues. g The effect of WDR1 knockdown on in vitro PDAC cell invasion was determined by transwell assay. h The effect of WDR1 knockdown on in vivo PDAC tumour metastasis; the sh-Ctrl and sh-WDR1 PANC1 cells were injected into the tail vein of BALB/c nude mice. After 30 days, lung metastasis was measured by an in vivo imaging system. i Representative immunohistochemical images of MMP9 staining from sh-Ctrl and sh-WDR1 lung metastasis tumour tissues. *P < 0.05, **P < 0.01.