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. 2020 Sep 15;11:4628. doi: 10.1038/s41467-020-18224-y

Fig. 1. Viscosity of poly-L-lysine coacervates is controlled by polymer length.

Fig. 1

a Brightfield image of polyK100/UTP condensates (10 mM Tris, pH 7.4). polyK concentration 6 mM per monomer and uridine triphosphate (UTP) 1.5 mM per monomer. b Widefield fluorescence image of polyK100/UTP condensate fusion (partitioned free Atto488 dye incorporated for enhanced visualization). c Confocal fluorescence image of polyK100/UTP droplet with 500 nm beads embedded (Red FluoSpheres, Invitrogen). Inset, representative 2D bead track. Scale bar 0.1 μm d Mean squared displacement (MSD) vs lag time for individual 500 nm beads in polyK100/UTP droplets. Inset, distribution of bead displacements at lag times = 0.5 s (red), 5 s (green), 10 s (blue). e MSD data for polyK10 (blue), polyK50 (red), and polyK100 (yellow) with UTP. Inset, viscosity as a function of polyK length. f Viscosity vs polyK length for polymers with UTP (blue), pU10 (green), and pU50 (yellow).