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. 2020 Sep 15;10:15106. doi: 10.1038/s41598-020-72208-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Details of a low-concentration trypsin-treated cartilage sample, its response to loading, and corresponding histological sections. Serial quantitative T1 (a), T1ρ (b), T2 (c), and T2* (d) maps as well as corresponding histological sections (e) of a representative sample before and after exposure to low concentration of trypsin at 0.1 mg/mL for 2 h. Histological assessment revealed intact tissue surface and tidemark as well as regular cellularity (e₁), but superficial Safranin-O de-staining indicative of proteoglycan depletion that was limited to the superficial tissue zone (e₂). Otherwise, image details as in Fig. 1.