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Microscope epifluorescence images showing semen from patients with epididymitis, (a) marking DNA with Sytox Orange (red); (b) Alexa Fluor 488 marking global histones (green); (c) Alexa Fluor 405 marking H4Cit3 (blue); and (d) a merge of channels. Magnification × 20. Graphs a and b show box plot with median and interquartile range differences of cells stained positive to global histones and citrullinated histones (H4Cit3), respectively. *P < 0.05
