Table 2.
Clinical parameters and outcome of ART treatment in patient with non-mosaic Klinefelter syndrome
| Patient | Cycle | COS protocol | Source of sperm(semen or testis) | Spermatozoa for ICSI (fresh or freeze/stock methods) | Spermatozoa status when performing ICSI | Oocyte activation | Injected MII oocyte (n) | 2PN fertilization rate (%) | Embryo cleaved number/rate (%) | Available embryo number/rate (%) | Embryo transferred (n) |
Clinical outcome (gender, gestational weeks, weight of newborn) |
niPGT-A | Prenatal screening | Birth defects | |||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Amount description | Usable spermatozoa characters after activation (mobility/morphology) | Spermatozoa activating methods | NIPT | Amniocentesis | ||||||||||||||
| 1 | 1 | Long | T | Freeze (cryopiece) | III (22sperms) | M/NM | AACR | N | 12 | 7 (58) | 7 (100) | 5 (71) | Fresh (10 III) | N | ||||
| Freeze |
Single live birth (Female, 39 weeks, 3900 g ) |
/ | Normal | / | Normal | |||||||||||||
| 2 | 1st | Mild | T | Fresh | I | M/AM | AACR | N | 2 | 2 (100) | 2 (100) | 2 (100) | Freeze | N | ||||
| 2nd | Mild | T | Freeze (straw) | I | M/AM | AACR | N | 4 | 4 (100) | 3 (75) | 3 (100) | Freeze |
Single live birth (Female, 38 + 3 weeks, 3250 g ) |
/ | Normal | / | Normal | |
| 3 | 1 | Antagonist | T | Freeze (LSL) | III |
IM/AM (ICSI cancel) |
AACR、HOS | |||||||||||
| 4 | 1 | Antagonist | T | Freeze (straw) | I | M/NM | AACR | N | 18 | 15 (83) | 15 (100) | 3 (20) | Fresh (6IV, 6IV) | N | ||||
| Freeze (4BC) | N | |||||||||||||||||
| 5 | 1 | Antagonist | T | Freeze (straw) | I | M/NM | AACR | N | 17 | 14 (82) | 14 (100) | 9 (64) | Fresh (8I, 10II) |
Twin live birth (Male, 37 weeks, 2900 g/2250 g) |
/ | / | / | Normal |
| 6 | 1 | Antagonist | T | Freeze (straw) | II | M/NM | AACR | N | 12 | 11 (92) | 10 (91) | 10 (91) | Fresh (8I, 8I) |
Single live birth (Male, 40 + 2 weeks, 4100 g ) |
/ | Normal | / | Normal |
| 7 | 1 | Antagonist | T | Freeze (straw) | II | M/NM | AACR | N | 18 | 12 (67) | 12 (100) | 11 (92) | Freeze | N | ||||
| 8 | 1 | Antagonist | T | Freeze (cryopiece) |
III (3 sperms) |
IM/AM (ICSI cancel) |
AACR、HOS | |||||||||||
| 9 | 1 | Antagonist | T | Fresh (overnight) Freeze (LSL) | IV |
IM/AM (ICSI cancel) |
AACR、HOS | |||||||||||
| 10 | 1st | Antagonist | T | Freeze (cryopiece) | III (12 sperms) |
IM/AM (ICSI cancel) |
AACR、HOS | |||||||||||
| 2nd | Fresh | IV |
No spermatozoa (ICSI cancel) |
AACR | ||||||||||||||
| 11 | 1st | Antagonist | T | Freeze (LSL) | IV | IM/NM | AACR、HOS | Y | 8 | 3 (38) | 3 (100) | 2 (67) | Fresh D2 (6II, 4III) | N | ||||
| 2nd | Antagonist | T | Fresh | IV |
IM/AM (ICSI cancel) |
AACR、HOS | ||||||||||||
| 12 | 1 | Antagonist | T | Fresh | IV |
IM/AM (ICSI cancel) |
AACR、HOS | |||||||||||
| 13 | 1 | Antagonist | T | Freeze (LSL) | I | M/NM | AACR | N | 3 | 1 (33) | 1 (100) | 1 (100) | Fresh (8I) | Miscarriage (4 weeks) | ||||
| 14 | 1 | Antagonist | T | Freeze (straw) | II | M/NM | AACR | N | 19 | 11 (58) | 11 (100) | 10 (91) | Freeze | Miscarriage (42 days) | ||||
| Freeze |
Single live birth (Female, 39 + 8 weeks, 3150 g ) |
Normal | Normal | / | Normal | |||||||||||||
| 15 | 1 | Antagonist | T | Fresh | IV | IM/NM | AACR、HOS | Y | 12 | 1 (8) | 0 | 0 | 0 | |||||
| 16 | 1 | Antagonist | T | Fresh | II | M/NM | AACR | N | 10 | 5 (50) | 5 (100) | 2 (40) | Freeze | N | ||||
| 17 | 1 | Antagonist | T | Freeze (straw) | I | M/NM | AACR | N | 3 | 0 | 0 | 0 | 0 | |||||
| 18 | 1 | Antagonist | T | Fresh | IV | IM/NM | AACR | Y | 11 | 5 (45) | 5 (100) | 4 (80) | Fresh (8I, 8I) | N | ||||
| Freeze | N | |||||||||||||||||
| 19 | 1 | Antagonist | T | Fresh | II | M/NM | AACR | Y | 3 | 2 (67) | 2 (100) | 2 (100) | Fresh (12IV, 8III) | N | ||||
| 20 | 1 | Antagonist | T | Freeze (straw) | I | M/NM | AACR | N | 16 | 9 (56) | 9 (100) | 9 (100) | Freeze |
Single live birth (female、33 + 6 weeks, 3350 g ) |
/ | Normal | / | Normal |
| 21 | 1 | Antagonist | T | Fresh | III | M/AM | AACR | N | 16 | 6 (38) | 6 (100) | 3 (50) | Freeze |
Twin live birth (Male, 37 weeks, 2550 g/3100 g) |
/ | / | / | Normal |
| 22 | 1 | Antagonist | T | Fresh | III | M/AM | AACR | N | 21 | 17 (81) | 17(100) | 11 (65) | Freeze |
Single live birth (Male, 39 + 5 weeks, 3400 g ) |
/ | Normal | / | Normal |
| 23 | 1 | Antagonist | T | Fresh | IV | IM/AM | AACR、HOS | Y | 5 | 3 (60) | 0 | 0 | 0 | |||||
| 24 | 1st | Antagonist | T | Freeze (straw) | III |
M/AM (ICSI cancel) |
||||||||||||
| 2nd | Fresh | III | M/AM | AACR | Y | 24 | 18 (75) | 10 (56) | 8 (44) | Freeze (10II, 8III) | N | |||||||
| Freeze | Pregnancy (16 weeks) | / | Normal | |||||||||||||||
| 25 | 1 | Antagonist | T | Fresh | IV | IM/AM | AACR | Y | 10 | 8 (80) | 6 (75) | 2 (11) |
Fresh (6III, 8III) |
Ectopic pregnancy | / | |||
| 26 | 1 | Antagonist | T | Fresh | II | M/AM | AACR | N | 10 | 5 (50) | 5 (100) | 2 (40) |
Fresh (6II, 6III) |
N | ||||
| 27 | 1 | Antagonist | T | Freeze (LSL) | I | M/NM | AACR | N | 13 | 9 (69) | 9 (100) | 9 (100) | Fresh (4I) | Pregnancy (35 + 2 weeks) | / | Normal | ||
| 28 | 1 | Antagonist | T | Fresh | I | M/NM | AACR | N | 11 | 3 (27) | 3 (100) | 3 (100) | Freeze | Pregnancy (19 + 5 weeks) | / | Normal | ||
| 29 | 1 | Antagonist | T | Fresh | II | M/NM | AACR | N | 8 | 7 (88) | 7 (100) | 2 (29) |
Fresh (8I, 8II) |
N | ||||
| 30 | 1 | Antagonist | T | Freeze (LSL) | IV | IM/NM | AACR | Y | 21 | 15 (71) | 11 (73) | 2 (13) | Freeze | N | ||||
| 31 | 1 | Antagonist | T | Fresh | II | M/AM | AACR | Y | 20 | 14 (70) | 12 (86) | 2 (14) | Freeze | N | ||||
IM, immobility; AM, abnormal morphology; NM, normal morphology; AACR, artificial activation with chemical reagents; niPGT-A, noninvasive preimplantation genetic testing for aneuploidy; NIPT, non invasive prenatal DNA testing. Amount description was defined as following: 100–150 μl centrifugation deposit from semen or testis sample was loaded on circular droplet (diameter 40 mm) with about 1000 low power fields (LP) with × 200 magnification; I, 1 sperm/10 LP (total > 100 spermatozoa ); II, 1 sperm/20 LP (total 10–50 spermatozoa ); III, 1 sperm/100 LP (total 1–10 spermatozoa); IV, no motile spermatozoa or no spermatozoa