Table 4.
Clinical parameters and outcome after intracytoplasmic sperm injection with vitrified spermatozoa cryopreservation in cryopiece and umssc system
| Patient | Diagnosis | Cycle | Spermatozoa origin | Single spermatozoa cryopreservation type | No. motile spermatoza/no.cryopiece | No. retrieved/no. vitrified spermaozoa (%) |
No. motile spermatozoa/no. retrieved spermatozoa (%) |
Clinic procedure | Oocyte activation | Injected MII oocyte | 2PN fertilization [n (%)] |
Embryo cleaved [n (%)] | Available embryo | Outcome (pregnancy date) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | NOA | 1 | m-TESE | Cryopiece | 20/2 | 18/20 (90) | 10/18 (56) | ICSI | No | 6 | 5 (83) | 5 (100) | 4 | Single live birth (2015–08–31) |
| 2 | Oligo | 1 | Ejaculate | Cryopiece | 40/2 | 30/40 (75) | 12/30 (40) | ICSI | No | 11 | 8 (73) | 7 (88) | 3 | Twin live birth (2015–09–24) |
| 3 | NOA | 1 | m-TESE | Cryopiece | 26/1 | 22/26 (85) | 10/22 (45) | ICSI | No | 8 | 5 (63) | 4 (80) | 1 | No pregnancy |
| 4 | NOA | 1 | m-TESE | Cryopiece | 20/1 | 18/20 (90) | 6/18 (33) | ICSI | No | 5 | 4 (80) | 3 (75) | 2 | Single live birth (2016–09–12) |
| 5 | NOA (KS) | 1 | m-TESE | Cryopiece | 22/1 | 18/22 (82) | 3/18 (17) | ICSI | No | 12 | 7 (58) | 7 (100) | 5 | Single live birth (2016–05–8) |
| 6 | NOA | 1 | m-TESE | Cryopiece | 25/1 | 18/25 (72) | 3/18 (17) | ICSI | No | 2 | 1 (50) | 1 (100) | 1 | No pregnancy |
| 7 | Oligo | 1 | Ejaculate | Cryopiece | 45/2 | 35/45 (78) | 10/35 (29) | ICSI | No | 18 | 3 (17) | 2 (67) | 2 | No pregnancy |
| 2 | Ejaculate | Cryopiece | 39/2 | 30/39 (77) | 9/30 (30) | ICSI | No | 9 | 3 (33) | 2 (67) | 2 | No pregnancy | ||
| 8 | Oligo | 1 | Ejaculate | Cryopiece | 21/1 | 16/21 (76) | 10/16 (63) | ICSI | No | 10 | 9 (90) | 9 (100) | 6 | Single live birth (2016–08–13) |
| 9 | Oligo | 1 | Ejaculate | Cryopiece | 33/2 | 25/33 (76) | 5/25 (20) | ICSI | No | 5 | 3 (60) | 3 (100) | 2 | No pregnancy |
| 10 | NOA (KS) | 1 | m-TESE | Cryopiece | 3/1 | 3/3 (100) | 0/3 | Donor semen | ||||||
| 11 | Oligo | 1 | Ejaculate | Cryopiece | 8/1 | 8/8 (100) | 6/8 (75) | ICSI | No | 6 | 3 (50) | 2 (67) | 0 | No pregnancy |
| 12 | NOA (KS) | 1 | m-TESE | Cryopiece | 20/1 | 12/20 (60) | 0/20 | Oocyte cryopreservation | ||||||
| 13 | NOA | 1 | m-TESE | Cryopiece | 11/1 | 7/11 (64) | 4/7 (57) | ICSI | No | 4 | 3 (75) | 3 (100) | 3 | No pregnancy |
| 14 | Oligo | 1 | Ejaculate | Cryopiece | 27/2 | 21/27 (78) | 8/21 (38) | ICSI | No | 8 | 6 (75) | 3 (50) | 3 | Single live birth (2017–11–10) |
| 15 | NOA | 1 | m-TESE | Cryopiece | 6/1 | 5/6 (83) | 0/6 (0) | ICSI | No | 5 | 2 (40) | 0 (0) | 0 | No pregnancy |
| 16 | Oligo | 1 | Ejaculate | Cryopiece | 21/1 | 21/21 (100) | 14/21 (67) | ICSI | No | 14 | 5 (36) | 5 (100) | 2 | No pregnancy |
| 17 | Oligo | 1 | Ejaculate | Cryopiece | 22/3 | 17/22 (77) | 7/17 (41) | ICSI | No | 7 | 3 (43) | 2 (67) | 1 | No pregnancy |
| 18 | Oligo | 1 | Ejaculate | Cryopiece | 14/1 | 13/14 (93) | 10/13 (77) | ICSI | No | 10 | 3 (30) | 3 (100) | 2 | No pregnancy |
| 19 | NOA | 1 | m-TESE | Cryopiece | 11/1 | 9/11 (82) | 3/9 (33) | ICSI | Yes | 5 | 4 (80) | 4 (100) | 3 | Single live birth (2018–03–26) |
| 20 | Oligo | 1 | Ejaculate | umSSC | 20/2 | 20/20 (100) | 14/20 (70) | ICSI | No | 8 | 5 (63) | 5 (100) | 2 | Single live birth (2018–07–05) |
| 21 | Oligo | 1 | m-TESE | Cryopiece | 7/1 | 5/7 (71) | 1/5 (20) | ICSI | No | 1 | 0 (0) | 0 (0) | 0 | No pregnancy |
| 22 | Oligo | 1 | Ejaculate | Cryopiece | 15/1 | 11/15 (73) | 7/11 (64) | ICSI | No | 14 | 9 (64) | 9 (100) | 7 | Single live birth (2018–11–01) |
| 23 | Oligo | 1 | Ejaculate | Cryopiece | 11/1 | 7/11 (64) | 3/7 (43) | ICSI | Yes | 5 | 5 (100) | 5 (100) | 2 | No pregnancy |
| 24 | Oligo | 1 | Ejaculate | Cryopiece | 12/1 | 9/12 (75) | 1/9 (11) | ICSI | Yes | 9 | 2 (22) | 2 (100) | 2 | Singleton ongoing pregnancy (2018–11–08) |
| 25 | Oligo | 1 | Ejaculate | umSSC | 22/2 | 20/22 (100) | 10/20 (50) | ICSI | No | 10 | 7 (70) | 7 (100) | 3 | Twins ongoing pregnancy (2019–01–8) |
| 26 | Oligo | 1 | Ejaculate | Cryopiece | 47/2 | 38/47 (81) | 7/38 (18) | ICSI | Yes | 8 | 6 (75) | 6 (100) | 4 | No pregnancy |
| 27 | Oligo | 1 | Ejaculate | Cryopiece | 12/1 | 9/12 (75) | 4/9 (44) | ICSI | Yes | 6 | 5 (83) | 4 (80) | 2 | No pregnancy |
umSSC, ultramicro single spermatozoa cryopreservation system