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. 2020 Aug 29;29(10):1425–1432. doi: 10.1007/s10068-020-00795-7

Table 1.

Analytical condition of HPLC with diode-array detection (DAD) for the determination of quantification of phenolic compounds from lemon myrtle leaves

Instrument Agilent 1200 series HPLC system
Detector Diode array at 257, 280, 325 and 365 nm
Column YMC-Triart C18 column (4.6 mm × 250 mm)
Column temp. 35 °C
Flow rate 0.8 mL/min
Mobile phase 2% formic acid in water (A) and 2% formic acid in methanol (B) was used as follows: water containing 2% acetic acid (water / acetic acid 98:2, (A)) and methanol (B), the composition gradient was: 95% (A), 5% (B) for 2 min; 25% (B) until 10 min; after, 40, 50, 60, 70, and 80% (B) every 10 min, respectively.
Injection vol. 10 μL