Table 1.
Instrument | Agilent 1200 series HPLC system |
Detector | Diode array at 257, 280, 325 and 365 nm |
Column | YMC-Triart C18 column (4.6 mm × 250 mm) |
Column temp. | 35 °C |
Flow rate | 0.8 mL/min |
Mobile phase | 2% formic acid in water (A) and 2% formic acid in methanol (B) was used as follows: water containing 2% acetic acid (water / acetic acid 98:2, (A)) and methanol (B), the composition gradient was: 95% (A), 5% (B) for 2 min; 25% (B) until 10 min; after, 40, 50, 60, 70, and 80% (B) every 10 min, respectively. |
Injection vol. | 10 μL |