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. 2020 Jul 17;37(9):2199–2209. doi: 10.1007/s10815-020-01891-7

Table 3.

miRNAs detected (Ct < 30) displayed as number of samples, where the miRNA was detected

miRNA/SnoRNA Control media (n = 3) Control MOPS-buffered media (n = 1) Spent media (n = 10) Blastocoel fluid (n = 7)
hsa-miR-92a-3p 3 1 3 5
hsa-miR-124-3p 3 1 10 7
hsa-miR-196a-5p 3 1 7 7
hsa-miR-214-3p 3 1 6 6
hsa-miR-22-3p 0 1 3 0
hsa-miR-142-5p 0 0 0 0
hsa-miR-375 0 1 0 1
hsa-miR-10a-5p 0 0 0 1
hsa-miR-100-5p 0 0 0 1
hsa-miR-137 0 0 1 0
hsa-miR-15a-5p 0 1 0 1
hsa-miR-99a-5p 0 0 0 1
hsa-miR-23b-3p 0 0 1 0
hsa-let-7c-5p 0 0 0 1
hsa-let-7a-5p 0 0 0 1
hsa-let-7b-5p 1 0 0 0
SNORD68 0 1 0 0

Control media were from the same batch as the culture media and cultured alongside the embryos. Several embryos could share a control if from the same batch and cultured the same day. MOPS-buffered media was taken directly from the bottle