Fig. 2.

Validation of the methylation level of imprinted gene DMR in semen samples analysed by BSAS. a–c The methylation level of CpG sites in the DMR of H19 (a), GNAS XL (b), SNRPN (c) and MEG8 (d). The x-axis represents the chromosomal location. e Comparisons of the DNA methylation levels of DMR of GNSA XL and three CpG sites (Chr19:2021940, 2021986 and 2022014) from H19 DMR and MEG8 DMR in the semen groups. *P < 0.05 vs. normozoospermia group. Significance was determined using a binary logistic regression adjusted by age