Table 1.
Sample parameters and global DNA methylation levels
| Normozoospermia | Asthenospermia | Oligospermia | Oligoasthenospermia | Deformity | |
|---|---|---|---|---|---|
| Number of samples | 8 | 16 | 3 | 11 | 2 |
| Age (year) | 30.00 ± 1.67 | 36.63 ± 2.48 | 32.67 ± 4.33 | 35.27 ± 1.95 | 36.50 ± 3.50 |
| Sperm concentration (× 106/ml) | 113.40 ± 21.95 | 53.14 ± 9.78* | 5.06 ± 1.30* | 8.79 ± 1.42* | 79.50 ± 24.90 |
| Total motility (%) | 66.51 ± 5.56 | 18.43 ± 2.78* | 45.10 ± 4.03 | 24.10 ± 2.57* | 52.10 ± 21.50 |
| Progressive motility (%) | 49.85 ± 4.07 | 12.45 ± 2.09* | 40.67 ± 4.81 | 19.24 ± 2.78* | 38.65 ± 22.15 |
| Nonprogressive motility (%) | 16.66 ± 2.81 | 5.98 ± 1.28* | 4.43 ± 1.79* | 4.04 ± 0.97* | 13.45 ± 0.65 |
| Sperm vitality (%) | 70.99 ± 3.52 | 38.82 ± 2.09* | 52.10 ± 7.13* | 43.06 ± 1.92* | 64.10 ± 16.10 |
| Number of samples analysed by RRBS | 8 | 4 | 3 | 2 | 2 |
| DNA methylation-whole genome (%) | 40.91 ± 2.41 | 37.84 ± 2.63 | 47.81 ± 4.67 | 40.56 ± 7.25 | 48.39 ± 6.90 |
| DNA methylation-gene body (%) | 40.19 ± 2.41 | 36.35 ± 2.67 | 46.43 ± 4.94 | 39.12 ± 7.53 | 47.32 ± 7.60 |
| DNA methylation-promoter (%) | 13.54 ± 1.22 | 11.05 ± 1.25 | 16.84 ± 3.07 | 12.66 ± 3.09 | 16.85 ± 4.54 |
| DNA methylation-CpG island (%) | 6.22 ± 0.22 | 5.30 ± 0.35* | 7.49 ± 1.23 | 6.21 ± 0.32 | 5.74 ± 0.40 |
| DNA methylation-3′-UTR (%) | 41.42 ± 2.24 | 38.44 ± 2.36 | 47.28 ± 4.61 | 40.40 ± 6.92 | 47.21 ± 6.94 |
| DNA methylation-5′-UTR (%) | 21.61 ± 2.15 | 17.80 ± 2.10 | 26.59 ± 4.58 | 20.10 ± 6.02 | 27.50 ± 7.37 |
| DNA methylation-exon (%) | 24.71 ± 1.49 | 20.11 ± 1.51 | 27.32 ± 4.14 | 22.83 ± 3.99 | 27.26 ± 6.02 |
| DNA methylation-intergenic (%) | 49.59 ± 2.29 | 51.82 ± 2.10 | 59.77 ± 2.50* | 55.07 ± 2.93 | 58.23 ± 1.61 |
| DNA methylation-intron (%) | 50.15 ± 2.31 | 47.31 ± 2.78 | 56.29 ± 4.15 | 49.71 ± 7.93 | 57.24 ± 6.28 |
*P value ≤ 0.05
RRBS, reduced representation bisulfite sequencing; UTR, untranslated region