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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: J Allergy Clin Immunol. 2020 Mar 16;146(4):884–893.e5. doi: 10.1016/j.jaci.2020.02.032

Figure 1. Omeprazole blocks IgE-induced mast cell degranulation, cytokine secretion and transcriptional changes.

Figure 1.

A) Representative histogram and bar plots for LAMP-1 expression by BMMCs following sensitization with IgE anti-TNP, treatment with drug vehicle (DMSO) or omeprazole, and challenge with TNP-OVA for 10 minutes. B) Histamine release into culture supernatant from IgE anti-TNP sensitized BMMCs following activation with TNP-OVA for 5 minutes. C) LAMP-1 expression of BMMCs following activation with adenosine. D) Heatmap of transcriptional changes in IgE sensitized BMMCs pre-treated with and without omeprazole followed by antigen stimulation for two hours. Direct mRNA counts as measured by the nCounter® Digital Analyzer System (NanoString) were normalized to internal positive, negative and housekeeping gene controls and presented as standard deviation from the row mean. E-H) Release of cytokines (IL-4, IL-6, IL-13, TNF-α) by IgE-sensitized BMMCs following omeprazole treatment and antigen stimulation for six hours. Statistical analysis by one-way analysis of variance. Data are shown for one experiment representative of two independent experiments for LAMP1, histamine and cytokine secretion assay; transcriptional profiling by NanoString was performed once. *P<0.05, **P<0.01, ***P<0.001, and ****P<0.0001