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. 2020 Sep 15;10:15119. doi: 10.1038/s41598-020-71865-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Sema3A restrains the size of synapses. (A) Gut explants cocultured with eGFP- or Sema3A-expressing COS-7 cells were immunolabeled with synapsin 1 and TuJ1 antibodies. Images at the bottom represent higher magnification of the boxed region. Scale bar 35 µm. (B,C) Quantification of the number of synapsin 1 clusters per 100 µm² of Tuj1 labeling (B) and area of synapsin 1 clusters (C). Data are mean ± SEM (n = 5); *p = 0.032, Mann–Whitney test.