THir (green) and DAPI (blue) stained coronal sections of cave (left; a, d) and surface (right; b, e) Astyanax. THir labeled somata had significantly larger (p < 0.001) diameters in cave Astyanax in the locus coeruleus (LC; d-f), but not in the posterior tuberal nucleus (PTN; a-c). The box plots in the right column (c, f) show the distributions of THir somata diameters of cave (pink) and surface (blue) Astyanax for that row. Black dots are the mean diameter, middle white line is the median, limits of the colored box indicate quartiles, and vertical white lines extend to the minimum and maximum diameters.