Figure 1. Presence of fibrocytes in the lungs of cystic fibrosis patients.
(A) Frozen lung sections from non-CF (n=4) and CF (n=6) individuals were co immunostained using CD45 and vimentin. Images are representative of each group. Arrows indicating CD45 & vimentin double-positive cells. (60X, Scale bar: 50μm) (B) Percent of vimentin-positive cells were quantified from each group using metamorph imaging software. (C) Percent fibrocytes (CD45+ and Vimentin+) were calculated using metamorph imaging software. DAPI stained cells were quantified for total cell number. *P < 0.05, **P < 0.005. Unpaired t-test.