Table 4.
Evaluation of the accuracy and precision of the plate count and TEMPO® methods at concentrations close to the acceptance criteria for human milk before pasteurization.
| Expected | Measured | CV (%) | Accuracy¥ | |
|---|---|---|---|---|
| concentration | concentration | (%) | ||
| (log CFU/ml) | (log CFU/ml) | |||
| Plate count method | ||||
| Total aerobic flora | 5.3 | 5.2 ± 0.1 | 1.7 | 98.1 |
| Enterobacteriaceae | 4.0 | 4.0 ± 0.1 | 1.4 | 100.0 |
| S. aureus | 3.0 | 2.7 ± 0.1 | 2.0 | 90.0 |
| Bacillus cereus (spores) | 1.0 | 0.8 ± 0.6 | 81.6 | 80.0 |
| TEMPO® method | ||||
| Total aerobic flora | 5.3 | 5.3 ± 0.1 | 2.9 | 100.0 |
| Enterobacteriaceae | 4.0 | 4.1 ± 0.1 | 3.0 | 102.5 |
| S. aureus | 3.0 | 2.5 ± 0.1 | 5.3 | 83.3 |
| Bacillus cereus (spores) | 1.0 | 1.2 ± 0.1 | 9.1 | 120.0 |
¥
Accuracy = (measured concentration/expected concentration) ×100 (n = 3).
Coefficient of variation = (standard deviation/mean) ×100 (n = 6).