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. 2020 Sep 2;11:1859. doi: 10.3389/fimmu.2020.01859

Figure 5.

Figure 5

(A) Quantification of STAT5 activity in functional subsets of CMV-specific T cells. STAT5 activity was determined by quantifying STAT5 phosphorylation in the functional subsets of CMV-specific T cells, and is expressed as the MFI relative to the non-functional subset (n = 4). (B,C) Reduction in polyfunctional cell cytokine expression (B) and proliferation (C) with the use of pathway-specific inhibitors Rapamycin (200 nM) and STAT5 inhibitor (CAS 285986-31-4, 200 μM) (n = 4). For cytokine expression, the data are presented as the percent-change in number of polyfunctional T cells relative to DMSO control (0.1% v/v). For proliferation, the data are presented as the division index (DI) for all remaining CMV-reactive T cells (i.e., expressing any type 1 cytokine), as the use of STAT5 inhibitor completely abolished the ability to detect polyfunctional T cells (n = 4). Statistical testing: ϕp < 0.005; *p < 0.05.