Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Sep 2;8:524676. doi: 10.3389/fbioe.2020.524676

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Hao, Suo, Lin, Chen, Zhou, Liu, Cui and Zhou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Construction of superfolder green fluorescence protein (sfGFP) insertion in sacA. (A) Scheme showing the procedures for gene insertion into Bacillus subtilis 168 by AIO system. (B) cPCR results shown that 9% (lane 5 and lane 11) of colonies had the sfGFP insertion mutant. (C) Scheme showing the procedures for gene insertion into B. subtilis 168 by CCB-CIGE system. (D) CIGE enables highly efficient sfGFP insertion mutation in the B. subtilis 168 strain. The sacA gene was replaced by the sfgfp gene. The efficiency for sfgfp gene insertion was 17/23 in the B. subtilis 168 strain. Prolonged incubation time under selective pressure increased the mutation efficiency to 82%.