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. 2020 Sep 2;11:1298. doi: 10.3389/fpls.2020.01298

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Copyright © 2020 Wang, Arling, Hoerster, Ryan, Wu, Lowe, Gordon-Kamm, Jones, Chilcoat and Anand

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The different stages in rapid maize transformation and heat shock treatment. (A) immature zygotic embryos are isolated and infected with Agrobacterium tumefaciens, (B) transgenic somatic embryos are placed for 3 weeks on selection media based on selectable marker used (HRA or NPTII), (C) somatic embryos are heat shock treated and transferred to maturation media, (D) transgenic plants are regenerated with or without selection pressure for 2 weeks and, (E) regenerated plants are placed on a rooting media for 2–3 weeks.