Table 3.

Transformation results from excision-activated marker gene selection using either the Glb1_pro or the Ole_pro driving Cre expression using construct design described in Figure 2 .

Promoter	Embryostransformed	T0 plants	T0 transformation frequency (% ± SE)	Total single copy events	Excised single copy, backbone-free events	Excision frequency (%)	Quality event (%)	Usable events (%)
Glb1	126	57	44.7 (2.8)a	19	13	68.4	13.3	5.6
Ole	112	45	40.2 (1.9)a	15	8	53.3	8.8	3.6

Data from two independent transformers was used to determine T0 transformation frequency. Quality events (QE) were identified as single copy, backbone-free, and morphogenic gene-free (excised). The excision frequency was determined as the ratio of the number of excised single-copy events relative to the total single-copy events. The number QEs was divided by the total number of events recovered to calculate the QE frequency. The usable event (UE) frequency is a measure of the number of acceptable transgenic events per 100 embryos that was determined as the product of QE frequency and transformation frequency. Mean values followed by the same letter are not statistically different from each other at the significance level of 0.05.

Data presents the T0 transformation frequency, qPCR detection of the number of excised events and the quality event frequency in maize inbred HC69.