Table 4.

Transformation results from excision-activated marker gene selection using Glbpro driving Cre expression using construct design described in Figure 2 .

Inbred	Embryos transformed	T0 plants	T0 transformation (% ± SE)	Total Single copy events	Excised single copy, backbone-free events	Excision frequency (%)	Quality event (%)	Usable events (%)
HC69	393	196	49.9 (3.9)a	31	17	54.8	8.7	4.3
PH85E	363	47	12.9 (1.3)c	22	13	59.0	27.7	3.6
PH84Z	367	105	28.6 (2.5)b	12	7	58.3	6.7	1.9

Data from two independent transformers was used to determine T0 transformation frequency. The quality events (QE) were identified as single copy, backbone-free, and morphogenic gene-free (excised). The excision frequency was determined as the ratio of the number of excised single-copy events relative to the total single-copy events. The number QEs was divided by the total number of events recovered to calculate the QE frequency. The usable event (UE) frequency is a measure of the number of acceptable transgenic events per 100 embryos that was determined as the product of QE frequency and transformation frequency. Mean values followed by the same letter are not statistically different from each other at the significance level of 0.05.

Data presents the T0 transformation frequency, qPCR detection of the number of excised events and the quality event frequency in three maize inbreds (HC69, PH85E, and PH84Z).