TABLE 1.
Strains used in this study.
| Strains | Description | Source or reference |
| E. coli JM109 | General cloning host | Our lab |
| E. coli ET12567 (pUZ8002) | Cmr, Kmr, donor strain for conjugation | Our lab |
| S. albus J1074 | Host for heterologous expression of gene cluster | Makitrynskyy et al., 2020 |
| S. diastatochromogenes 1628 | Wild-type strain, toyocamycin producer | CGMCC 2060 |
| S. albus J1074-TC | Strain S. albus J1074 integrated with native toy cluster | This work |
| 1628-Δcluster | Toy gene cluster disruption mutant, derived from 1628 strain | This work |
| 1628-Δcluster/pSET152 | Mutant 1628-Δcluster with integrative plasmid pSET152 | This work |
| 1628-Δcluster/pSET152::ncluster | Toy cluster complemented strain, mutant 1628-Δcluster with integrative plasmid pSET152::ncluster | This work |
| 1628-pSET152 | Strain S. diastatochromogenes 1628 with integrative vector pSET152 | This work |
| 1628-TC | Strain S. diastatochromogenes 1628 with integrative vector pSET152::ncluster, harboring extra copy of native toy cluster | This work |
| 1628-EC | Strain S. diastatochromogenes 1628 with integrative vector pSET152::ecluster, harboring extra copy of engineered toy cluster | This work |
| 1628-SC | Strain S. diastatochromogenes 1628 with integrative vector pSET152::scluster, harboring extra copy of engineered toy cluster | This work |