TABLE 1.
Bacterial strains, plasmids, and oligonucleotides used in this study.
| Bacterial strains used in this study | |||
| Strain | Organism | Genotype | Source |
| JDW2901 | B. subtilis | 3610 ΔzpdN ΔSPβΔPBSX ΔcomI | Daniel Kearns |
| BKK34780 | B. subtilis | 168 ΔnahA::kanR | BGSC |
| JDW3014 | B. subtilis | JDW2901 ΔywaCΔyjbM | This work |
| JDW4009 | E. coli | DH5α/pDR110-ywaC | This work |
| JDW4011 | E. coli | DH5α/pDR110-ywaCD87G | This work |
| JDW4017 | B. subtilis | JDW2901 ΔywaCΔyjbMΔrelA::mls amyE::Pspank-ywaC | This work |
| JDW4019 | B. subtilis | JDW2901 ΔywaCΔyjbMΔrelA::mls amyE::Pspank-ywaCD87G | This work |
| JDW4064 | B. subtilis | JDW2901 ΔywaCΔyjbMΔrelA::mls amyE::Pspank-ywaCΔnahA::kanR | This work |
| JDW4066 | B. subtilis | JDW2901 ΔywaCΔyjbMΔrelA::mls amyE::Pspank-ywaCD87GΔnahA::kanR | This work |
| Plasmids used in this study | |||
| Plasmid | Genotype | Source | |
| pDR110 | amyE::Pspank amp spc | David Rudner | |
| pJW300 | pJW239/ΔyjbM I-SceI site amp cat | Lab stock | |
| pJW306 | pJW299/ΔywaC I-SceI site amp cat | Lab stock | |
| pJW512 | pDR154/amyE::Pxyl-ywaC | Lab stock | |
| pJW516 | pDR154/amyE::Pxyl-ywaCD87G | Lab stock | |
| pJW731 | pDR110/amyE::Pspank-ywaC | This work | |
| pJW733 | pDR110/amyE::Pspank-ywaCD87G | This work | |
| pSS4332 | oriU Pamy-I-sceI kan | Scott Stibitz | |
| Oligonucleotides used in this study | |||
| Oligo | Sequence (5′ 3′) | ||
| oJW358 | ATGTATGGCCGGAACTGAAG | ||
| oJW359 | CGGTCGCTGTATCTGTGAAA | ||
| oJW902 | AAAGAGGCGCTTTTGACGTG | ||
| oJW903 | TTGTTGACCCGGGACATGGA | ||
| oJW904 | CGTCCTCATACGTTAACCGC | ||
| oJW905 | GGGCTATCAAAAGGACTTTACCG | ||
| oJW1519 | TCATCCATCATACATCCTCCTTCTGTCGACTTAATTAAACCACTTTG | ||
| oJW3382 | GCTCAAAGTATTCTTCAAGCGAGAG | ||
| oJW3383 | CATTCCACTTCATGACGTAAGAGG | ||
| oJW3495 | ACATGCATGCAAAGGAGGTGTACATATGGATTTATCTGTAACACATATGGACG | ||
| oJW3496 | ACATGTCGACTTAATCCACTTCTTTCTTAATCCCCAGC | ||