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Gating strategy for detection of MVs in plasma samples. (A) Size calibration beads ranging from 0.3 to 1 μm were used to define a MV gate. (B) AV staining was further included to specifically detect MVs due to their PS surface expression. EDTA control was included to block specific binding of AV to PS. Counting beads were used to quantify the amount of shed MVs in samples as described in the text.
