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. 2020 Sep 2;11:1789. doi: 10.3389/fimmu.2020.01789

Figure 3.

Figure 3

C5a alters MV shedding in PMNs. (A) PMNs were stimulated for 0, 10, 30, 60, and 240 min with C5a (100 ng/ml) or LPS (5 μg/ml) and supernatants were analyzed by FACS. Untreated cells served as control. n = 10, One-Way ANOVA followed by Student-Neuman-Keuls post-hoc testing was performed for each time point, *p < 0.05. (B) MVs from supernatants were further analyzed for expression of C5aR1 by FACS. n = 3 per group, *p < 0.05. (C) Heat map and gene list of selected and differentially regulated genes upon C5a treatment in in neutrophils after 1 h (n = 3 per group).