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C5aR1 expression and MV shedding after incubation of PMNs with serum obtained from PT patients (S PT) and healthy donors (S Healthy). PMNs were incubated in HBSS++ buffer supplemented with 20% of pooled human serum from PT patients or from healthy donors for 1 h. (A) Quantification of C5aR1 expression on PMNs and (B) C5aR1+ MV counts in 10 μl of PMN supernatants after incubation. n = 4, *p < 0.05.
