Table 2.

Structures of allergen-antibody complexes by X-ray crystallography.

Allergen in complex with IgG antibody construct
Allergen	Allergen source	Allergen expression system	Antibody	Antibody expression system	PDB code
Api m 2	Honeybee	Insect cells (high five)	Fab; mIgG1 mAb 21E11	Mus musculus hybridoma cells	2J88 (48)*
Bet v 1	Birch	E. coli	Fab′; mIgG1 mAb BV16	Mus musculus hybridoma cells	1FSK (49)*
Bla g 2	German cockroach	P. pastoris	Fab′, mIgG1 mAb 7C11	Mus musculus hybridoma cells	2NR6 (50) (51)*
Bla g 2	German cockroach	P. pastoris	Fab, mIgG1 mAb 4C3	Mus musculus hybridoma cells	3LIZ (52)*
Der f 1	House dust mite	D. farinae mite culture	Fab; mIgG1 mAb 4C1	Mus musculus hybridoma cells	5VPL (53) (54)*
Der p 1	House dust mite	D. pteronyssinus mite culture	Fab; mIgG1 mAb 4C1	Mus musculus hybridoma cells	1) 3RVW (53) 2) 3RVX (53) (55)*
Der p 1	House dust mite	D. pteronyssinus mite culture	Fab; mIgG1 mAb 5H8	Mus musculus hybridoma cells	4PP1 (56) (57)*
Der p 1	House dust mite	D. pteronyssinus mite culture	Fab; mIgG1 mAb 10B9	Mus musculus hybridoma cells	4PP2 (56) (57)* (55)*
Der p 2	House dust mite	P. pastoris	Fab; mIgG1 mAb 7A1	Mus musculus hybridoma cells	6OY4 (58)* (59)*
Fel d 1	Cat	CHO	Fab; IgG4 mAb REGN1909	CHOc	5VYF (60)
Gal d 4 (lysozyme)	Chicken	Not specified (most likely Gallus gallus)		E. coli (1FDL) or Mus musculus hybridoma cells	(1) (42) (2) (43) (3) 3HFM (44) (4) 1FDL (45) (5) 1MLC (46) (6) 1YQV (47)
Gal d 4 (lysozyme)#	Chicken	Not specified	Human VH domain; VH H04 Phage displayed	E. coli BL21 Gold	(1) 4PGJ (61) (2) 4U3X (61)
Phl p 7	Timothy grass	E. coli BL21 star DE3	Fab; hIgG1 mAb 102.1F10 was expressed based on a hIgG4 that was generated from matched heavy- and light-chain sequences by single B cell cloning from allergic individuals	FreeStyle 293F	5OTJ (62)
Allergen in complex with IgG antibody constructs containing human IgE variable regions
Bos d 5	Cow	E. coli	Fab; hIgG1 mAb D1: Cκ and CH1 of IgG1 cloned with IgE VH/VL isolated from human IgE derived from a combinatorial library	E. coli RV308	2R56 (63)
Phl p 2	Timothy grass	E. coli BL21	Fab; hIgG1 mAb huMab2: Cκ and CH1 of IgG1 cloned with IgE VH/VL isolated from human IgE derived from a combinatorial library	CHO-K1	2VXQ (64)*

^*Manuscripts that report inhibition of IgE antibody binding by the antibody used in the X-ray crystal structure (or viceversa).

^#Only selected complexes with lysozyme are listed. For example, complexes of human V_H domains with lysozyme were chosen to compare them with complexes formed by Fabs.