Figure 4.

RvE1 reduces pro-inflammatory mRNA expression in BMDMs with LPS stimulation in vitro. BMDMs were treated with 10 nM RvE1 or vehicle after 2 h LPS (0.1 μg/ml) stimulation. Real-time quantitative PCR of (A) IL-6, (B) IL-1β, (C) CCL2, and (D) TNFα expression, relative to 18S rRNA. Each analysis (A–D) is from a single experiment and is representative of six separate experiments. Data are expressed as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 vs. LPS + Vehicle group. US, unstimulated.