Table 1.
Primers Used for Semi-Quantitative RT-PCR
| Gene | Forward Primer Sequence (5ʹ to 3ʹ) | Reverse Primer Sequence (5ʹ to 3ʹ) | Product Size (bp) |
|---|---|---|---|
| GAPDH | GAAGGTGAAGGTCGGAGT | GAAGATGGTGATGGGATTTC | 226 |
| VDR | CTTCAGGCGAAGCATGAAGC | CCACCATCATTCACACGAACTGG | 128 |
| CYP24A1 | GCTTCTCCAGAAGAATGCAGGG | CAGACCTTGGTGTTGAGGCTCT | 125 |
| FN 1 | CCAACTGGTAACCCTTCC | CCAACACTGGGTTGCTGA | 156 |
| LUM | AACATACCAACTGTCAATGAAAACC | TGCCATCCAAACGCAAATGCTTG | 125 |
| BGN | TTGAACCTGGAGCCTTCGATGG | TTGGAGTAGCGAAGCAGGTCCT | 150 |
| MMP2 | AGCGAGTGGATGCCGCCTTTAA | CATTCCAGGCATCTGCGATGAG | 137 |
| TIMP1 | GGAGAGTGTCTGCGGATACTTC | GCAGGTAGTGATGTGCAAGAGTC | 100 |
| COL5A1 | GGAGATGATGGTCCCAAAGGCA | CCATCATCTCCTTTGTCACCAGG | 118 |
| CCL2 | CCCCAGTCACCTGCTGTTAT | TGGAATCCTGAACCCACTTC | 171 |
| CCL5 | CGCTGTCATCCTCATTGCTA | GAGCACTTGCCACTGGTGTA | 147 |
| CCL11 | AATGTCCCCAGAAAGCTGTG | TCAGGCTCTGGTTTGGTTTC | 163 |
| CCND1 | CAATGACCCCGCACGATTTC | AAGTTGTTGGGGCTCCTCAG | 208 |
| BCL2 | CTTTGAGTTCGGTGGGGTCA | GGGCCGTACAGTTCCACAAA | 162 |
| BAX | TCAGGATGCGTCCACCAAGAAG | TGTGTCCACGGCGGCAATCATC | 103 |